Use of dialysis to separate small and large molecules
Gel filtration chromatography
Molecular mass determination by gel filtration chromatography
Some commonly used gel filtration materials
Ion-exchange chromatography with stepwise elution
Device for generating a linear concentration gradient
Molecular formulas of cellulose-based ion exchangers
Some biochemically useful ion exchangers
Affinity chromatography
Covalent linking of ligand to agarose
Derivatization of epoxy-activated agarose
Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose
Experimental arrangement for paper chromatography
Two-dimensional paper chromatography
Paper electrophoresis
Apparatus for slab gel electrophoresis
Diagram of a disc electrophoresis apparatus
Polymerization of acrylamide and N,Ną-methylenebisacrylamide to form a cross-linked polyacryl-amide gel
SDS-PAGE
Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE
General formula of the ampholytes used in isoelectric focusing
Isoelectric points of several common proteins
Purification of rat liver glucokinase
PPT Slide
An enzyme-linked immunosorbent assay (ELISA)
Solubilities of several proteins in ammonium sulfate solutions
Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type
Solubility of b-lactoglobin as a function of pH at several NaCl concentrations
Protein crystals
Agarose gel electrophoretogram of a double helical DNA
Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs
Detection of proteins by immunoblotting
Two-dimensional (2D) gel electrophoresis
Sedimentation coefficients in Svedbergs (S) for some biological materials
A selection of preparative ultracentrifuge rotors
Zonal ultracentrifugation
Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm
Isopycnic ultracentrifugation
Physical constants of some proteins
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