The Center for Zebrafish Research, located in the basement of the Biology Department Building at the University of Notre Dame, is a versatile and efficient 3,000 square foot facility. The fish are maintained in open racks that are connected by 5 independent life support systems. The racks have a flexible arrangement that permits the use of different size tanks (ranging from 1 liter to 5 liters) which allow anywhere from one pair of adults up to 50 adults to be maintained in a single tank. The total capacity of the facility is nearly 150,000 adult zebrafish. The racks also contain five light-controlled chambers which allow us to manipulate the light cycle of several tanks so that we may either dark-adapt zebrafish or collect embryos throughout the day. The Center is staffed by two accredited animal technicians who care for the fish, clean the tanks, and maintain the facility 365 days a year.
The Center currently houses three different wild-type stocks (AB, Tubingen, and WIK), 20 different mutants (10 that were generated at the University of Notre Dame), and over 25 different transgenic lines, most of which express EGFP in specific cell types and were generated at Notre Dame. We also have both wild-type and mutant stocks of medaka. On average, each month the zebrafish facility ships 5 mutant and/or transgenic stocks to zebrafish labs throughout the world.
Holding Room 1: Shows dark box enclosures
Holding Room 1
Holding Room 2
Holding Room 3
Cage Washing Room
Karen feeing AB stock fish in the Breeding room