This course is roughly divided into two parts. The first half will cover principles of cell and developmental biology that guide current approaches in tissue engineering and regenerative medicine. An emphasis will be placed on the computational and quantitative analysis of biological processes such as cell-cell signaling and morphogenesis. The second half covers techniques involved in cultivating cells for applications in recombinant protein production as well as the design of bioartificial organs and regenerative therapeutics. Optimization techniques for culture medium development will also be covered.