University of Notre Dame College of Engineering

Zartman Lab

Quantitative Analysis of Pattern Formation, Growth and Regeneration

Tissue and cell culture optimization

Tissue and cell culture optimization

tissue and cell culture optimizationIn recent years, insect cells have become important for heterologous recombinant protein production using the baculovirus expression system. Developing a better quantitative understanding of the metabolism and kinetics of insect cell growth will significantly improve the production process of novel therapeutics and vaccines using insect cell culture.

A greater challenge is identifying the requirements for growing organs in vitro, which have more stringent needs than established cell lines. Current research focuses on identifying extrinsic growth factors and improving media for the short-term culture of Drosophila wing imaginal discs, an established model for studying the genetics and biophysics of growth control.

Future efforts will be directed toward designing next-generation insect culture media by identifying novel growth factors and developing tools for growing organs in vitro.