Table of Contents
“L23 Protein Functions as a Chaperone Docking Site on the Ribosome”
Overview
Chaperones and Folding
Chaperone Pathway in Bacteria
Trigger Factor (TF)
Significance
A Few Questions
TF Signature
TF Signature and Mutants
TF Signature Mutants
FRK/AAA Mutant Association with Ribosomes
FRK/AAA Mutant Association with Ribosomes
TF Signature Mutants
D42C Mutant Association and Crosslinking with Ribosomes
D42C Mutant Association and Crosslinking with Ribosomes
Interaction is Specific
L23 and L29
L23 and L29 Deletion Mutants
L23 and L29 Deletion Mutants
L23 and L29 Deletion Mutants
L29 and TF Binding
TF Remains Associated to L29-Deficient Ribosomes
TF Can Rebind to L29-Deficient Ribosomes
L23 Deletion and Mutants
L23 Region 1 and 2 Mutants
L23 Region 1 and 2 Mutants
L23 Mutants and TF Binding
L23 Mutants and TF Binding
L23 Mutants and TF Binding
L23 Mutants and TF Binding
TF Interacts Directly with L23
TF Interacts Directly with L23
TF • Nascent Polypeptide Interaction and L23
In Vitro Transcription/ Translation System
Crosslinking
Identifying Crosslink Results
L23 is Required for TF • ICDH Interaction
TF-Ribosome Interaction and In Vivo Protein Folding
TF-Ribosome Interaction and in vivo Protein Folding
TF-Ribosome Interaction and in vivo Protein Folding
The Big Picture
Conclusions
Future Directions
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