Methods: Markers of neurogenesis
Neuronal nuclear antigen (NeuN)
Neuron-specific nuclear protein (vs cytoplasmic or cell-surface antigen) observed in invertebrates
Recognized with a mAb in standard IHC
Specifically reactive for post-migratory (late maturity) neurons
No non-specific (ex. glial) reaction within NS; no non-neuronal detection
Doesn’t detect all types of neurons, but most