Pulsed H/D Exchange
X-H + D2O _ X-D + HOD
Used to follow the time course of protein folding by 2D NMR
a. Denatured protein in D2O
b. Dilute with H2O and allow to fold for time tf
c. Increase pH to initiate D-H exchange (10-40 ms)
d. Lower pH; allow to completely fold
e. Determine which amide protons are protonated and deuterated
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